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polyacrylamide (pa) “easy coat” substrates  (Matrigen Life Technologies)


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    Matrigen Life Technologies polyacrylamide (pa) “easy coat” substrates
    Polyacrylamide (Pa) “Easy Coat” Substrates, supplied by Matrigen Life Technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polyacrylamide+substrates/polyacrylamide++pa++%E2%80%9Ceasy+coat++substrates/pm38095311-42-4-11
    Average 90 stars, based on 1 article reviews
    polyacrylamide (pa) “easy coat” substrates - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Microscopy:

    Article Title: A novel mechanoeffector role of fibroblast S100A4 in myofibroblast transdifferentiation and fibrosis.
    Article Snippet: TGF (2 ng/ml) was added and the live cells were imaged using Leica SP8 inverted confocal microscope with a 20x/0.4NA long-working distance objective (24 hours, images taken every 30 minutes for persistent SA; 6 hours, images taken every 15 minutes when SA not present, 37°C, 5% CO2, 95% humidity). .. Traction Force Microscopy – Polyacrylamide substrates (shear moduli 25 kPa) embedded with 0.2 μm yellow/green fluorospheres (Matrigen) were incubated with 10 ug/ml fibronectin for 2 hours. ..

    Shear:

    Article Title: A novel mechanoeffector role of fibroblast S100A4 in myofibroblast transdifferentiation and fibrosis.
    Article Snippet: TGF (2 ng/ml) was added and the live cells were imaged using Leica SP8 inverted confocal microscope with a 20x/0.4NA long-working distance objective (24 hours, images taken every 30 minutes for persistent SA; 6 hours, images taken every 15 minutes when SA not present, 37°C, 5% CO2, 95% humidity). .. Traction Force Microscopy – Polyacrylamide substrates (shear moduli 25 kPa) embedded with 0.2 μm yellow/green fluorospheres (Matrigen) were incubated with 10 ug/ml fibronectin for 2 hours. ..

    Incubation:

    Article Title: A novel mechanoeffector role of fibroblast S100A4 in myofibroblast transdifferentiation and fibrosis.
    Article Snippet: TGF (2 ng/ml) was added and the live cells were imaged using Leica SP8 inverted confocal microscope with a 20x/0.4NA long-working distance objective (24 hours, images taken every 30 minutes for persistent SA; 6 hours, images taken every 15 minutes when SA not present, 37°C, 5% CO2, 95% humidity). .. Traction Force Microscopy – Polyacrylamide substrates (shear moduli 25 kPa) embedded with 0.2 μm yellow/green fluorospheres (Matrigen) were incubated with 10 ug/ml fibronectin for 2 hours. ..



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    Image Search Results


    Schematic illustration of experimental process to find novel genes involved in biophysical stimulation. (A) The four sample conditions used in this study: shCTRL and shDDR2 cells cultured on 2 and 20 kPa collagen‐coated polyacrylamide gel substrates. (B) Experimental process of RNA‐seq: a 24‐h incubation of the cells on PAA gels followed by cellular RNA extraction, library construction, sequencing, and data analysis. This figure is created with BioRender.com .

    Journal: FEBS Open Bio

    Article Title: DDR2 signaling and mechanosensing orchestrate neuroblastoma cell fate through different transcriptome mechanisms

    doi: 10.1002/2211-5463.13798

    Figure Lengend Snippet: Schematic illustration of experimental process to find novel genes involved in biophysical stimulation. (A) The four sample conditions used in this study: shCTRL and shDDR2 cells cultured on 2 and 20 kPa collagen‐coated polyacrylamide gel substrates. (B) Experimental process of RNA‐seq: a 24‐h incubation of the cells on PAA gels followed by cellular RNA extraction, library construction, sequencing, and data analysis. This figure is created with BioRender.com .

    Article Snippet: Polyacrylamide gel substrates were prepared through the polymerization of acrylamide (Bio‐Rad, Waltham, MA, USA, Cat. No. 1610140) and bis‐acrylamide, with varying concentrations to achieve the desired stiffness levels (Table ).

    Techniques: Cell Culture, RNA Sequencing, Incubation, RNA Extraction, Sequencing